eukaryoticexpressionvector相关论文
Construction of pEYFP-hApelin-R and pRluc-hNTSRI-pcDNA3.1 eukaryotic expression vectors and their ex
Objective To construct expression vectors that enhanced yellow fluorescent protein (EYFP) fused with Apelin receptor (Ap......
目的：构建人骨形态发生蛋白（BMP）2的真核表达型载体，并利用其与壳聚糖制备纳米复合体。方法体外利用双酶切法将pMD18T-hBMP2-His质粒与......
Objective:To reconstruct pEGFP-C2-L539fs/47,a HERG nonsense mutant in eukaryotic expression plasmid,and observe the fusi......
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Vascular proliferation is one of the major causes for morbidity and mortality in diabetes. However, the cellular and mol......
The paper aimed at constructing the eukaryotic expression vector of CecropinB and transfecting it into the goat mammary ......
目的：通过构建过表达质粒并筛选稳定细胞株，研究SPATA5L1基因对KB细胞增殖和迁移的影响。方法 PCR扩增SPATA5L1基因全长，用载体pEX-1......
AIM: To construct a pEgr-IFNγ plasmid and to investigate its expression properties of interferon-γ (INF-γ) induced by......
AIM: To study the transcriptional regulation of human telomerase reverse transcriptase (hTERT) mRNA in normal human chol......
Contribution of C3d-P28 repeats to enhancement of immune responses against HBV-preS2/S induced by ge
AIM: To investigate whether P28 derived from C3d can enhance the immune response to HBV-preS2/S induced by directly inje......
AIM: To transfect murine angiostatin cDNA into human hepatocellular carcinoma cell line SMMC-7721 and to investigate its......
AIM: To establish a mice model of hepatitis B by using HBV-transgenic mice, and to transfer HBV-specific cytotoxic T lym......
Inhibitory effect of antisense vascular endothelial growth factor 165 eukaryotic expression vector o
AIM: To construct antisense VEGF165 eukaryotic expression vector PCDNA3-as-VEGF165 and to study its expression and effec......
Effect of NHE1 antisense gene transfection on the biological behavior of SGC-7901 human gastric carc
AIM: To study the effect of type 1 Na+/H+ exchanger (NHE1) antisense human gene transfection on the biological behavior ......
目的 构建人白细胞介素-10(hIL-10)高效真核表达载体,观察其在家兔滑膜细胞(RSCs)中表达.方法 提取人外周血单个核细胞(PBMCs)总RN......
目的 构建GPC3基因真核表达重组质粒,观察GPC3对生长因子FGF2、IGF2促进肝癌细胞增殖的影响.方法 自行设计引物从GPC3原核扩增重组......
目的 构建骨形成蛋白(BMP)-2、转化牛长因子(TCF)-β1双基因真核表达载体.方法 以pGEM-T-BMP-2及pGEM-T-TGF-β1两个质粒为模板,分......
目的 构建大鼠淀粉样前体蛋白(APP)695基因真核细胞荧光表达载体,进行细胞转染、表达,为阿尔茨海默病(AD)治疗药物的研究提供一种......
目的:构建含TCRγV1基因的重组表达载体.方法:从人淋巴瘤Jurkat细胞中提取总RNA,反转录合成cDNA克隆入VR1012质粒;转化感受态细菌E......
本研究利用真核表达载体ｐＲＣ／ＲＳＶ，将位于ｐＢＳ－ＳＫ质粒中的ＣａｌｐａｓｔａｔｉｎｃＤＮＡ以正反两个方向构入载体；经酶切分析，构建的质粒结构正确。所构建质粒ｐＲＣ／ＲＳＶ正义、反义ＣａｌｐａｓｔａｔｉｎｃＤＮＡ两个表达载体......
